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Catalog Number:
47754
CAS Number:
2044-85-1
2',7'-Dichlorofluorescein diacetate
Grade:
suitable for fluorescence
Purity:
≥ 98% (HPLC)
Synonym(s):
Diacetyldichlorofluorescein, DCFHDA
Documents
$104.00 /250MG
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Product Information

2',7'-Dichlorofluorescein diacetate is a versatile fluorescent dye widely utilized in biological and chemical research. This compound is particularly valued for its ability to permeate cell membranes, making it an excellent tool for cellular imaging and tracking. Once inside the cell, it is hydrolyzed by esterases to release the highly fluorescent 2',7'-dichlorofluorescein, which can be used to monitor various cellular processes, including apoptosis, oxidative stress, and membrane integrity. Researchers often employ this compound in flow cytometry and fluorescence microscopy to gain insights into cellular functions and dynamics.

In addition to its applications in cell biology, 2',7'-Dichlorofluorescein diacetate is also used in environmental monitoring and analytical chemistry. Its ability to detect reactive oxygen species (ROS) makes it a valuable indicator in studies related to oxidative stress in various biological systems. The compound's high sensitivity and specificity allow for precise measurements, making it a preferred choice for researchers looking to explore the intricate details of cellular behavior and environmental interactions.

Synonyms
Diacetyldichlorofluorescein, DCFHDA
CAS Number
2044-85-1
Purity
≥ 98% (HPLC)
Grade
suitable for fluorescence
Molecular Formula
C24H14Cl2O7
Molecular Weight
485.27
MDL Number
MFCD00037501
Melting Point
232 - 247 °C
Appearance
White to off-white to beige powder
Conditions
Store at 2 - 8 °C
General Information
Synonyms
Diacetyldichlorofluorescein, DCFHDA
CAS Number
2044-85-1
Purity
≥ 98% (HPLC)
Grade
suitable for fluorescence
Molecular Formula
C24H14Cl2O7
Molecular Weight
485.27
MDL Number
MFCD00037501
Melting Point
232 - 247 °C
Appearance
White to off-white to beige powder
Conditions
Store at 2 - 8 °C
Properties
Additional property information coming soon!
-
Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

2',7'-Dichlorofluorescein diacetate is widely utilized in research focused on:

  • Cell Viability Assays: This compound is commonly used in assays to assess cell viability and proliferation, providing researchers with a reliable method to evaluate the health of cultured cells.
  • Fluorescence Microscopy: It serves as a fluorescent dye in microscopy, allowing for the visualization of cellular components and processes, which is crucial in fields like cell biology and pharmacology.
  • Drug Development: In pharmaceutical research, it is used to study drug effects on cellular functions, helping in the identification of potential therapeutic compounds.
  • Biochemical Pathway Analysis: The compound aids in tracking metabolic pathways in live cells, providing insights into cellular responses to various stimuli or treatments.
  • Environmental Monitoring: It can be employed in environmental science to detect and quantify biological activity in water samples, contributing to ecological studies and assessments.

Citations