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Catalog Number:
39628
4-Nitrophenyl phosphate (Ready-to-use solution) [for ELISA]
Synonym(s):
p-Nitrophenyl phosphate (Ready-to-use solution), pNPP (Ready-to-use solution)
Documents
$184.28 /100ML
Pack Size Availability Price
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Product Information

4-Nitrophenyl phosphate is a versatile and essential reagent widely utilized in enzyme-linked immunosorbent assays (ELISA) and other biochemical applications. This ready-to-use solution serves as a substrate for alkaline phosphatase, facilitating the detection of various biomolecules through colorimetric assays. Its ability to produce a measurable color change upon enzymatic hydrolysis makes it invaluable in both research and diagnostic laboratories.

The compound's high purity and stability ensure reliable results, making it an ideal choice for researchers seeking accuracy in their assays. Additionally, 4-Nitrophenyl phosphate is recognized for its compatibility with various buffers and conditions, enhancing its applicability across different experimental setups. Its use in ELISA not only streamlines the detection process but also improves the sensitivity of the assays, allowing for the quantification of low-abundance targets. By incorporating this reagent into your workflow, you can achieve consistent and reproducible results, essential for advancing your research or diagnostic capabilities.

Synonyms
p-Nitrophenyl phosphate (Ready-to-use solution), pNPP (Ready-to-use solution)
Appearance
Colorless, slightly orange or yellow clear liquid
Conditions
Store at ≤ -20 °C
General Information
Synonyms
p-Nitrophenyl phosphate (Ready-to-use solution), pNPP (Ready-to-use solution)
Appearance
Colorless, slightly orange or yellow clear liquid
Conditions
Store at ≤ -20 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

4-Nitrophenyl phosphate (Ready-to-use solution) is widely utilized in research focused on:

  • Enzyme-linked Immunosorbent Assays (ELISA): This compound serves as a substrate in ELISA tests, allowing researchers to quantify proteins or antibodies in samples effectively.
  • Biochemical Research: It is used in various biochemical assays to study enzyme activity, particularly in phosphatase assays, providing insights into enzyme kinetics.
  • Diagnostics Development: The compound plays a crucial role in developing diagnostic tests for diseases, helping to identify biomarkers through sensitive detection methods.
  • Pharmaceutical Research: In drug development, it aids in screening potential drug candidates by evaluating their interactions with specific enzymes.
  • Environmental Monitoring: Used in assays to detect phosphatase activity in environmental samples, it helps assess the health of ecosystems and the presence of pollutants.

Citations