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Catalog Number:
38781
CAS Number:
77-86-1
Trizma base
Grade:
suitable for cell culture
Purity:
≥ 99.9% (Assay by titration on dried basis)
Synonym(s):
Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol
Documents
$55.28 /100G
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Product Information

Trizma base, also known as tris(hydroxymethyl)aminomethane, is a versatile buffering agent widely utilized in biochemical and molecular biology applications. With its unique ability to maintain a stable pH environment, Trizma base is essential in various laboratory protocols, including protein purification, enzyme assays, and cell culture. Its excellent solubility in water and compatibility with biological systems make it a preferred choice for researchers seeking reliable results in sensitive experiments.

In addition to its buffering capabilities, Trizma base serves as a stabilizing agent for enzymes and proteins, enhancing their activity and longevity during experimental procedures. It is particularly beneficial in the preparation of electrophoresis buffers and in the formulation of biological reagents. By providing a consistent pH, Trizma base helps to minimize variability in experimental outcomes, making it an indispensable tool in research and development across the life sciences.

Synonyms
Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol
CAS Number
77-86-1
Purity
≥ 99.9% (Assay by titration on dried basis)
Grade
suitable for cell culture
Molecular Formula
C4H11NO3
Molecular Weight
121.1
MDL Number
MFCD00004679
PubChem ID
6503
Melting Point
168 - 172 °C
Density
1.353 g/mL
Appearance
White crystalline powder
Boiling Point
219 - 220 °C/10 mmHg
Conditions
Store at RT
General Information
Synonyms
Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol
CAS Number
77-86-1
Purity
≥ 99.9% (Assay by titration on dried basis)
Grade
suitable for cell culture
Molecular Formula
C4H11NO3
Molecular Weight
121.1
MDL Number
MFCD00004679
PubChem ID
6503
Melting Point
168 - 172 °C
Density
1.353 g/mL
Appearance
White crystalline powder
Boiling Point
219 - 220 °C/10 mmHg
Conditions
Store at RT
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Trizma base is widely utilized in research focused on various applications:

  • Buffer Solutions: Commonly used in biochemistry and molecular biology, Trizma base helps maintain pH stability in buffer solutions, which is crucial for enzyme reactions and protein stability.
  • Cell Culture Media: It is an essential component in cell culture media, providing a stable environment for the growth of cells in vitro, which is vital for pharmaceutical research and development.
  • Electrophoresis: In laboratories, it is frequently employed in electrophoresis techniques, aiding in the separation of biomolecules such as DNA and proteins, thereby facilitating genetic and proteomic studies.
  • Pharmaceutical Formulations: Trizma base is used in the formulation of various pharmaceuticals, enhancing the solubility and stability of active ingredients, which improves drug efficacy.
  • Analytical Chemistry: It serves as a reagent in analytical chemistry for titrations and other quantitative analyses, providing accurate results in determining concentrations of various substances.

Citations