Bicine is a versatile zwitterionic buffer widely utilized in biochemical and molecular biology applications. With its unique structure, Bicine offers excellent buffering capacity in the physiological pH range, making it ideal for use in various laboratory settings, including protein purification, enzyme assays, and electrophoresis. Its low ionic strength and minimal interference with biological systems enhance its suitability for sensitive experiments, particularly in the preparation of biological samples and the stabilization of proteins and nucleic acids.

Researchers appreciate Bicine for its compatibility with a wide range of biomolecules, allowing for optimal conditions in experiments involving enzymes and other proteins. Its ability to maintain pH stability under varying temperatures and ionic conditions further underscores its importance in laboratory protocols. Bicine is also recognized for its low toxicity, making it a safer alternative to traditional buffers, which is particularly beneficial in cell culture and other sensitive applications.