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Catalog Number:
21690
CAS Number:
16096-97-2
L-Dithiothreitol
Purity:
≥ 95% (Assay by titration)
Synonym(s):
(-)-1,4-Dithio-L-threitol, (-)-Cleland's reagent, (2R,3R-(-)-2,3-Dihydroxy-1,4-butanedithiol
Documents
$110.00 /250MG
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Product Information

L-Dithiothreitol is a powerful reducing agent widely utilized in biochemical and molecular biology applications. This compound is particularly valued for its ability to maintain proteins in a reduced state, preventing oxidation during various experimental procedures. Its unique structure allows it to effectively break disulfide bonds, making it an essential reagent in protein purification, enzyme assays, and the stabilization of sensitive biomolecules. Researchers often employ L-Dithiothreitol in the preparation of samples for electrophoresis, as it enhances the resolution of proteins by ensuring they remain in their reduced form.

In addition to its role in protein chemistry, L-Dithiothreitol has shown potential in therapeutic applications, including its use in the treatment of oxidative stress-related conditions. Its ability to scavenge free radicals and protect cellular components from oxidative damage makes it a valuable compound in both research and clinical settings. With its broad range of applications and effectiveness in maintaining protein integrity, L-Dithiothreitol is an indispensable tool for scientists and industry professionals alike.

Synonyms
(-)-1,4-Dithio-L-threitol, (-)-Cleland's reagent, (2R,3R-(-)-2,3-Dihydroxy-1,4-butanedithiol
CAS Number
16096-97-2
Purity
≥ 95% (Assay by titration)
Molecular Formula
C4H10O2S2
Molecular Weight
154.25
MDL Number
MFCD00064305
PubChem ID
19001
Melting Point
48 - 53 ?C
Appearance
White to almost white crystal powder
Optical Rotation
[a]D20 = 22 - 28 ° (C=2.5 in MeOH)
Conditions
Store at 0 - 8 °C
General Information
Synonyms
(-)-1,4-Dithio-L-threitol, (-)-Cleland's reagent, (2R,3R-(-)-2,3-Dihydroxy-1,4-butanedithiol
CAS Number
16096-97-2
Purity
≥ 95% (Assay by titration)
Molecular Formula
C4H10O2S2
Molecular Weight
154.25
MDL Number
MFCD00064305
PubChem ID
19001
Melting Point
48 - 53 ?C
Appearance
White to almost white crystal powder
Optical Rotation
[a]D20 = 22 - 28 ° (C=2.5 in MeOH)
Conditions
Store at 0 - 8 °C
Properties
Additional property information coming soon!
-
Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

L-Dithiothreitol is widely utilized in research focused on:

  • Protein Stabilization: It is commonly used to maintain the reduced state of proteins during purification processes, preventing oxidation and preserving their functional integrity.
  • Cell Culture: In cell biology, it helps to reduce disulfide bonds in proteins, promoting cell viability and enhancing the growth of sensitive cell lines.
  • Biochemical Assays: Researchers use it in various assays to ensure accurate measurements of enzyme activity by keeping proteins in their active forms.
  • Antioxidant Applications: Its reducing properties make it effective in protecting cells from oxidative stress, which is crucial in studies related to aging and disease.
  • Drug Formulation: In pharmaceutical development, it serves as a stabilizing agent for certain drugs, improving their shelf life and efficacy.

Citations