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Catalog Number:
01899
CAS Number:
69-78-3
5,5'-Dithio-bis2-nitrobenzoic acid)
Purity:
≥ 99% (HPLC)
Synonym(s):
Ellman's reagent, 3-Carboxy-4-nitrophenyl disulfide, DTNB, Bis(3-carboxy-4-nitrophenyl) disulfide
Documents
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Product Information

5,5'-Dithio-bis(2-nitrobenzoic acid) is a versatile chemical compound widely recognized for its applications in biochemical research and analytical chemistry. This compound, also known as DTNB or Ellman's reagent, is primarily utilized for the quantification of thiol groups in proteins and peptides, making it an essential tool for researchers studying enzyme activity and protein structure. Its unique disulfide bond allows for the selective reaction with free thiols, resulting in a colorimetric change that can be easily measured, providing a straightforward method for assessing protein concentration and functionality.

In addition to its role in protein analysis, 5,5'-Dithio-bis(2-nitrobenzoic acid) has potential applications in drug development and the study of redox biology. Its ability to form stable complexes with metal ions also opens avenues for its use in various catalysis and sensor technologies. Researchers and industry professionals appreciate its reliability and efficiency, making it a preferred choice for those engaged in biochemical assays and related fields.

Synonyms
Ellman's reagent, 3-Carboxy-4-nitrophenyl disulfide, DTNB, Bis(3-carboxy-4-nitrophenyl) disulfide
CAS Number
69-78-3
Purity
≥ 99% (HPLC)
Molecular Formula
C14H8N2O8S2
Molecular Weight
396.35
MDL Number
MFCD00007140
PubChem ID
6254
Melting Point
240 - 245 °C (dec.)
Appearance
Light yellow to yellow powder
Conditions
Store in the dark at 0 - 8 °C
General Information
Synonyms
Ellman's reagent, 3-Carboxy-4-nitrophenyl disulfide, DTNB, Bis(3-carboxy-4-nitrophenyl) disulfide
CAS Number
69-78-3
Purity
≥ 99% (HPLC)
Molecular Formula
C14H8N2O8S2
Molecular Weight
396.35
MDL Number
MFCD00007140
PubChem ID
6254
Melting Point
240 - 245 °C (dec.)
Appearance
Light yellow to yellow powder
Conditions
Store in the dark at 0 - 8 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

5,5'-Dithio-bis(2-nitrobenzoic acid) is widely utilized in research focused on:

  • Biochemical Assays: This compound is commonly used as a reagent in biochemical assays to measure thiol concentrations, helping researchers understand cellular redox states.
  • Protein Labeling: It serves as a labeling agent for proteins, allowing scientists to track and study protein interactions and functions in various biological systems.
  • Drug Development: In pharmaceutical research, it aids in the development of drugs targeting oxidative stress-related diseases by providing insights into thiol-disulfide exchange reactions.
  • Environmental Monitoring: The compound is utilized in environmental chemistry to detect and quantify thiol compounds in samples, which is crucial for assessing pollution levels.
  • Analytical Chemistry: It plays a role in analytical methods for the detection of heavy metals, enhancing the sensitivity and specificity of tests in various industrial applications.

Citations