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Catalog Number:
00488
CAS Number:
67-42-5
Ethylene glycol-bis(b-aminoethyl ether)-N,N,N',N'-tetraacetic acid
Purity:
99 - 101% (Assay by titration)
Synonym(s):
EGTA, Ethylene-bis(oxyethylenenitrillo)tetraacetic acid
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Product Information

Ethylene glycol-bis(b-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) is a high-purity chelating agent commonly used in biochemical and molecular biology applications. With its unique structure, EGTA has a selective binding affinity for calcium ions over magnesium ions, making it particularly valuable in studies where calcium ion regulation is critical. This reagent is essential for maintaining controlled environments in cell culture and protein isolation protocols.

Synonyms
EGTA, Ethylene-bis(oxyethylenenitrillo)tetraacetic acid
CAS Number
67-42-5
Purity
99 - 101% (Assay by titration)
Molecular Formula
C14H24N2O10
Molecular Weight
380.35
MDL Number
MFCD00004291
Melting Point
240 - 244 °C
Appearance
White powder
Conditions
Store at RT
General Information
Synonyms
EGTA, Ethylene-bis(oxyethylenenitrillo)tetraacetic acid
CAS Number
67-42-5
Purity
99 - 101% (Assay by titration)
Molecular Formula
C14H24N2O10
Molecular Weight
380.35
MDL Number
MFCD00004291
Melting Point
240 - 244 °C
Appearance
White powder
Conditions
Store at RT
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) is a high-purity chelating agent commonly used in biochemical and molecular biology applications. 

Applications and Uses:

  • Calcium Ion Chelation: Effectively binds calcium ions to regulate concentrations in biochemical assays and cellular experiments.
  • Biochemical Research: Used in protocols for protein purification, enzyme activity modulation, and buffer preparation.
  • Cellular Studies: Helps to stabilize cell membranes and prevent calcium-dependent processes during experiments.
  • Molecular Biology: Integral in maintaining specific ionic conditions in PCR, electrophoresis, and other molecular techniques.

Citations