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Catalog Number:
00459
CAS Number:
5704-04-1
N-trisHydroxymethyl)methylglycine
Purity:
≥ 99% (Titration: anhydrous basis)
Synonym(s):
N-[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine, Tricine
Documents
$20.00 /25G
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Product Information

N-tris(Hydroxymethyl)methylglycine, commonly known as Tris, is a versatile buffering agent widely utilized in biochemical and molecular biology applications. This compound is particularly valued for its ability to maintain stable pH levels in various laboratory settings, making it essential for experiments involving enzymes, proteins, and nucleic acids. Its unique structure allows it to effectively resist changes in pH, providing researchers with reliable conditions for optimal reactions. Tris is frequently employed in the preparation of electrophoresis buffers, cell culture media, and various biochemical assays, ensuring reproducibility and accuracy in experimental results.

In addition to its buffering capabilities, N-tris(Hydroxymethyl)methylglycine is recognized for its low toxicity and compatibility with a wide range of biological systems, making it an ideal choice for sensitive applications. Researchers appreciate its effectiveness in stabilizing proteins and nucleic acids during purification processes, which is crucial for downstream applications such as cloning and sequencing. With its proven track record in laboratories worldwide, Tris continues to be a fundamental reagent for scientists seeking reliable and efficient solutions in their research endeavors.

Synonyms
N-[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine, Tricine
CAS Number
5704-04-1
Purity
≥ 99% (Titration: anhydrous basis)
Molecular Formula
C6H13NO5
Molecular Weight
179.2
MDL Number
MFCD00004277
PubChem ID
79784
Melting Point
182-184°C (dec)
Appearance
White powder
Conditions
Store at RT
General Information
Synonyms
N-[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine, Tricine
CAS Number
5704-04-1
Purity
≥ 99% (Titration: anhydrous basis)
Molecular Formula
C6H13NO5
Molecular Weight
179.2
MDL Number
MFCD00004277
PubChem ID
79784
Melting Point
182-184°C (dec)
Appearance
White powder
Conditions
Store at RT
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

N-tris(Hydroxymethyl)methylglycine is widely utilized in research focused on:

  • Biotechnology: This compound serves as a buffering agent in various biological assays, helping to maintain pH stability which is crucial for enzyme activity and cell culture.
  • Pharmaceutical Development: It is used in drug formulation to enhance solubility and stability of active pharmaceutical ingredients, improving the efficacy of medications.
  • Analytical Chemistry: As a reagent, it aids in the detection and quantification of metal ions in complex samples, providing accurate results in environmental monitoring.
  • Food Industry: The compound acts as a stabilizer in food products, ensuring consistent quality and extending shelf life without compromising safety.
  • Cosmetics: It is incorporated into skincare formulations for its moisturizing properties, enhancing product performance and consumer satisfaction.

Citations