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Catalog Number:
00238
CAS Number:
369-07-3
2-Nitrophenyl-β-D-galactopyranoside
Purity:
≥ 99% (HPLC)
Synonym(s):
2-Nitrophenyl-β-D-galactoside
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Product Information

2-Nitrophenyl-b-D-galactopyranoside is a valuable compound widely utilized in biochemical research and enzymatic studies. This substrate is particularly important for the detection and quantification of β-galactosidase activity, making it an essential tool in molecular biology and biochemistry laboratories. Its unique structure allows for the release of a colored product upon enzymatic hydrolysis, facilitating easy monitoring of enzyme activity. Researchers often employ this compound in assays to study enzyme kinetics, gene expression, and metabolic pathways, providing insights into cellular processes and disease mechanisms.

Additionally, 2-Nitrophenyl-b-D-galactopyranoside is recognized for its stability and solubility in various solvents, which enhances its applicability in diverse experimental setups. Its use extends to the development of biosensors and diagnostic kits, where precise measurement of enzyme activity is crucial. By choosing this compound, researchers can achieve reliable results, contributing to advancements in biotechnology and pharmaceutical development.

Synonyms
2-Nitrophenyl-β-D-galactoside
CAS Number
369-07-3
Purity
≥ 99% (HPLC)
Molecular Formula
C12H15NO8
Molecular Weight
301.3
MDL Number
MFCD00063255
PubChem ID
219973
Melting Point
195 °C (dec)
Appearance
White to off-white crystalline powder
Optical Rotation
[a]D20 = -67 to -73 º (C=1 in H2O)
Conditions
Store at 0 - 8 °C
General Information
Synonyms
2-Nitrophenyl-β-D-galactoside
CAS Number
369-07-3
Purity
≥ 99% (HPLC)
Molecular Formula
C12H15NO8
Molecular Weight
301.3
MDL Number
MFCD00063255
PubChem ID
219973
Melting Point
195 °C (dec)
Appearance
White to off-white crystalline powder
Optical Rotation
[a]D20 = -67 to -73 º (C=1 in H2O)
Conditions
Store at 0 - 8 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

2-Nitrophenyl-b-D-galactopyranoside is widely utilized in research focused on:

  • Enzyme Activity Assays: This compound serves as a substrate for various glycosidases, allowing researchers to measure enzyme activity and understand their kinetics in biochemical studies.
  • Cellular Uptake Studies: It can be used to investigate the mechanisms of sugar transport in cells, providing insights into cellular metabolism and potential therapeutic targets.
  • Fluorescent Probes: When coupled with fluorescent tags, it can be employed in imaging studies to visualize cellular processes, enhancing the understanding of cellular dynamics.
  • Drug Development: The compound's ability to mimic natural substrates makes it valuable in the design of new drugs targeting glycosidases, contributing to advancements in pharmaceutical research.
  • Biochemical Pathway Analysis: It aids in the elucidation of metabolic pathways involving carbohydrates, helping researchers to map out complex biological processes.

Citations