TMB [for ELISA] is a ready-to-use solution that serves as a crucial substrate in enzyme-linked immunosorbent assays (ELISA), widely utilized in both clinical and research laboratories. This compound, also known as 3,3',5,5'-Tetramethylbenzidine, is characterized by its ability to produce a blue color upon oxidation, which can be quantitatively measured to determine the presence of specific antigens or antibodies. Its high sensitivity and stability make it an ideal choice for various applications, including diagnostics, biomarker discovery, and quality control in pharmaceutical manufacturing.

Researchers and industry professionals appreciate TMB for its ease of use and reliable performance, which can significantly enhance assay accuracy and reproducibility. Its compatibility with a range of enzyme labels, such as horseradish peroxidase (HRP), allows for versatile application across different assay formats. Moreover, TMB's rapid reaction kinetics and minimal background interference contribute to its effectiveness, making it a preferred choice for high-throughput screening and diagnostic testing.