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Catalog Number:
38781
CAS Number:
77-86-1
Base Trizma
Grade:
adapté à la culture cellulaire
Purity:
≥ 99,9 % (dosage par titrage sur une base sèche)
Synonym(s):
Trométamol, 2-Amino-2-(hydroxyméthyl)-1,3-propanediol
Documents
$55.28 /100 g
Taille
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Informations sur le produit

Trizma base, also known as tris(hydroxymethyl)aminomethane, is a versatile buffering agent widely utilized in biochemical and molecular biology applications. With its unique ability to maintain a stable pH environment, Trizma base is essential in various laboratory protocols, including protein purification, enzyme assays, and cell culture. Its excellent solubility in water and compatibility with biological systems make it a preferred choice for researchers seeking reliable results in sensitive experiments.

In addition to its buffering capabilities, Trizma base serves as a stabilizing agent for enzymes and proteins, enhancing their activity and longevity during experimental procedures. It is particularly beneficial in the preparation of electrophoresis buffers and in the formulation of biological reagents. By providing a consistent pH, Trizma base helps to minimize variability in experimental outcomes, making it an indispensable tool in research and development across the life sciences.

Numéro CAS 
77-86-1
Formule moléculaire
C 4 H 11 NON 3
Poids moléculaire 
121.1
Point de fusion 
168 - 172 °C
Informations générales
Numéro CAS 
77-86-1
Formule moléculaire
C 4 H 11 NON 3
Poids moléculaire 
121.1
Point de fusion 
168 - 172 °C
Propriétés
Informations complémentaires sur la propriété à venir prochainement !
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Sécurité et réglementation
Matières dangereuses
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Antibiotique
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Réglementé par la DEA
Non
Avertissements 
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Applications

Trizma base is widely utilized in research focused on various applications:

  • Buffer Solutions: Commonly used in biochemistry and molecular biology, Trizma base helps maintain pH stability in buffer solutions, which is crucial for enzyme reactions and protein stability.
  • Cell Culture Media: It is an essential component in cell culture media, providing a stable environment for the growth of cells in vitro, which is vital for pharmaceutical research and development.
  • Electrophoresis: In laboratories, it is frequently employed in electrophoresis techniques, aiding in the separation of biomolecules such as DNA and proteins, thereby facilitating genetic and proteomic studies.
  • Pharmaceutical Formulations: Trizma base is used in the formulation of various pharmaceuticals, enhancing the solubility and stability of active ingredients, which improves drug efficacy.
  • Analytical Chemistry: It serves as a reagent in analytical chemistry for titrations and other quantitative analyses, providing accurate results in determining concentrations of various substances.

Citations