EPPS, or 3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid, is a versatile zwitterionic buffer widely utilized in biological and biochemical research. Known for its exceptional buffering capacity in physiological pH ranges, EPPS is particularly effective in maintaining stable pH levels in various laboratory applications, including protein purification, enzyme assays, and cell culture. Its unique structure allows for minimal interference with biological systems, making it an ideal choice for sensitive experiments where pH stability is crucial.

Researchers appreciate EPPS for its low toxicity and compatibility with a wide range of biological molecules, which enhances its utility in drug formulation and development. Additionally, EPPS is often employed in the preparation of buffers for electrophoresis and chromatography, where precise pH control is essential for optimal separation and analysis. Its ability to function effectively in both aqueous and non-aqueous environments further expands its applicability in diverse scientific fields, from molecular biology to pharmaceuticals.