Hygromycin B from Streptomyces hygroscopicus powder is widely utilized in research focused on:

• Antibiotic Research: It serves as a potent antibiotic, particularly effective against a range of Gram-negative bacteria, making it invaluable in microbiological studies and antibiotic resistance research.
• Cell Culture Applications: Researchers use it as a selective agent in cell culture to maintain and select genetically modified cells, ensuring that only those expressing the hygromycin resistance gene survive.
• Gene Expression Studies: It aids in the study of gene expression by allowing scientists to create stable cell lines that express specific genes, facilitating the investigation of gene function and regulation.
• Plant Transformation: In plant biotechnology, hygromycin B is employed as a selection marker in the transformation of plants, helping researchers to identify successfully transformed plants in genetic engineering projects.
• Pharmaceutical Development: It is used in the development of new antibiotics and therapeutic agents, providing a basis for testing the efficacy of new compounds against resistant bacterial strains.