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Catalog Number:
00498
CAS Number:
25535-16-4
Propidium iodide
Purity:
≥ 95% (Assay by titration)
Documents
$51.18 /25MG
Pack Size Availability Price
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Product Information

Propidium iodide is a vital chemical compound widely utilized in molecular biology and cell biology applications. Known for its ability to intercalate with nucleic acids, it serves as a powerful fluorescent dye for staining dead cells in various assays, including flow cytometry and fluorescence microscopy. This compound is particularly effective in distinguishing live from dead cells, making it an essential tool in apoptosis studies and cell viability assessments. Its unique properties allow researchers to visualize cellular structures and monitor cellular processes, enhancing the understanding of complex biological systems.

In addition to its applications in cell viability assays, propidium iodide is also employed in DNA and RNA analysis, providing insights into genetic material integrity. Its compatibility with other fluorescent dyes allows for multiplexing in experiments, thereby increasing the efficiency of data collection. Researchers benefit from its high sensitivity and specificity, which facilitate accurate results in various experimental setups. With its broad range of applications in research and diagnostics, propidium iodide continues to be an indispensable resource for professionals in the life sciences.

CAS Number
25535-16-4
Purity
≥ 95% (Assay by titration)
Molecular Formula
C27H34N4I2
Molecular Weight
668.41
MDL Number
MFCD00011921
PubChem ID
24871268
Melting Point
220 - 235 ºC
Appearance
Red color powder
Conditions
Store at 0-8 °C
General Information
CAS Number
25535-16-4
Purity
≥ 95% (Assay by titration)
Molecular Formula
C27H34N4I2
Molecular Weight
668.41
MDL Number
MFCD00011921
PubChem ID
24871268
Melting Point
220 - 235 ºC
Appearance
Red color powder
Conditions
Store at 0-8 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Propidium iodide is widely utilized in research focused on:

  • Cell Viability Assays: It is commonly used to assess cell membrane integrity, allowing researchers to distinguish between live and dead cells in various biological studies.
  • Flow Cytometry: This compound is essential in flow cytometry applications, where it helps in the analysis of cell populations by staining DNA in dead cells, providing insights into cell health and apoptosis.
  • Fluorescent Microscopy: Propidium iodide is employed in fluorescent microscopy to visualize cellular structures and processes, particularly in studies involving cancer research and cell cycle analysis.
  • DNA Staining: It is used for staining nucleic acids in gel electrophoresis, facilitating the visualization of DNA bands, which is crucial for genetic research and diagnostics.
  • Antimicrobial Studies: This compound helps evaluate the effectiveness of antimicrobial agents by determining the viability of bacteria and fungi, aiding in the development of new treatments.

Citations