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Catalog Number:
00666
CAS Number:
1239-45-8
Bromure d'éthidium
Purity:
≥ 99 % (HPLC)
Synonym(s):
Bromure de 2,7-diamino-10-éthyl-9-phénylphénanthridinium, Bromure d'homidium
Hazmat
Documents
$22.03 /1G
Taille
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Informations sur le produit

Ethidium bromide is a powerful intercalating agent widely utilized in molecular biology and biochemistry for its ability to bind to nucleic acids. This compound is particularly renowned for its application in gel electrophoresis, where it serves as a fluorescent stain for DNA and RNA visualization. When exposed to ultraviolet light, ethidium bromide emits a bright orange fluorescence, making it an essential tool for researchers analyzing genetic material. Its effectiveness in detecting nucleic acids has made it a staple in laboratories for applications such as cloning, sequencing, and PCR analysis.

In addition to its role in nucleic acid visualization, ethidium bromide is also employed in various research settings to study cell viability and apoptosis. Its unique properties allow researchers to assess the integrity of cellular membranes, providing insights into cellular health and function. While handling ethidium bromide requires caution due to its mutagenic properties, its benefits in molecular research are unparalleled, making it a vital compound for professionals in the life sciences.

Numéro CAS 
1239-45-8
Formule moléculaire
C 21 H 20 BrN 3
Poids moléculaire 
394.32
Point de fusion 
260 - 262 ?C
Informations générales
Numéro CAS 
1239-45-8
Formule moléculaire
C 21 H 20 BrN 3
Poids moléculaire 
394.32
Point de fusion 
260 - 262 ?C
Propriétés
Informations complémentaires sur la propriété à venir prochainement !
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Sécurité et réglementation
Matières dangereuses
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Antibiotique
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Réglementé par la DEA
Non
Avertissements 
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Applications

Ethidium bromide is widely utilized in research focused on:

  • DNA Visualization: Commonly used in gel electrophoresis, it intercalates between DNA bases, allowing researchers to visualize DNA fragments under UV light, which is essential for genetic analysis and cloning.
  • Cell Viability Assays: This compound helps assess cell viability by distinguishing live cells from dead ones, making it valuable in toxicology and pharmacology studies.
  • RNA Analysis: Similar to its use with DNA, ethidium bromide can also be used to visualize RNA in gel electrophoresis, aiding in the study of gene expression and RNA integrity.
  • Microbial Studies: It serves as a selective agent in microbiology for isolating and identifying specific bacterial strains, enhancing the efficiency of research in microbiome studies.
  • Research on Drug Interactions: Ethidium bromide is used to study the binding properties of drugs to nucleic acids, providing insights into drug design and development in pharmaceutical research.

Citations