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Catalog Number:
38770
CAS Number:
5625-37-6
TUYAUX
Grade:
adapté à la culture cellulaire
Purity:
≥ 99 % (dosage par titration, HPLC)
Synonym(s):
Acide 1,4-pipérazinediéthanesulfonique, Pipérazine- N,N' -bis(acide 2-éthanesulfonique)
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Product Information

PIPES, or 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid, is a versatile buffering agent widely utilized in biological and biochemical research. This compound is particularly valued for its ability to maintain a stable pH in various experimental conditions, making it an essential component in cell culture and protein purification processes. PIPES is known for its low absorbance in the UV range, which enhances its suitability for spectrophotometric applications. Researchers often employ PIPES in electrophoresis and chromatography, where precise pH control is critical for optimal separation and analysis of biomolecules.

Additionally, PIPES is recognized for its compatibility with a wide range of biological systems, including proteins and nucleic acids, which allows for its use in diverse applications such as enzyme assays and molecular biology protocols. Its unique properties, including a pKa value that is ideal for physiological pH ranges, make it a preferred choice over other buffering agents. By incorporating PIPES into your research, you can ensure reliable results and enhanced reproducibility in your experiments.

Synonyms
Acide 1,4-pipérazinediéthanesulfonique, Pipérazine- N,N' -bis(acide 2-éthanesulfonique)
CAS Number
5625-37-6
Purity
≥ 99 % (dosage par titration, HPLC)
Grade
adapté à la culture cellulaire
Molecular Formula
C8H18N2O6S2
Molecular Weight
302.37
MDL Number
MFCD00006159
PubChem ID
79723
Melting Point
> 300 °C
Appearance
Poudre blanche
Conditions
Magasin chez RT
General Information
Synonyms
Acide 1,4-pipérazinediéthanesulfonique, Pipérazine- N,N' -bis(acide 2-éthanesulfonique)
CAS Number
5625-37-6
Purity
≥ 99 % (dosage par titration, HPLC)
Grade
adapté à la culture cellulaire
Molecular Formula
C8H18N2O6S2
Molecular Weight
302.37
MDL Number
MFCD00006159
PubChem ID
79723
Melting Point
> 300 °C
Appearance
Poudre blanche
Conditions
Magasin chez RT
Properties
Additional property information coming soon!
-
Safety and Regulations
Hazmat
Non
Antibiotic
Non
DEA-regulated
Non
Warnings
-
Applications

PIPES is widely utilized in research focused on:

  • Biological Buffers: PIPES serves as an effective buffering agent in biological and biochemical applications, maintaining pH stability in cell culture and enzyme reactions, which is crucial for accurate experimental results.
  • Protein Purification: It is commonly used in protein purification processes, particularly in chromatography techniques, helping to stabilize proteins and improve yield during purification.
  • Cell Biology: Researchers use PIPES in cell biology studies to create optimal conditions for cell growth and maintenance, ensuring that cellular processes are not disrupted by pH fluctuations.
  • Pharmaceutical Formulations: PIPES is incorporated into pharmaceutical formulations to enhance the solubility and stability of active ingredients, thereby improving drug efficacy.
  • Electrophoresis: In molecular biology, it is utilized in electrophoresis techniques, providing a stable environment for the separation of nucleic acids and proteins, which is essential for accurate analysis.