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Catalog Number:
00174
CAS Number:
7365-45-9
Acide 4-(2-hydroxyéthyl)-1-pipérazineéthanesulfonique
Purity:
99 - 101 % (dosage par titrage)
Synonym(s):
HEPES
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Informations sur le produit

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, commonly known as HEPE, is a versatile buffering agent widely utilized in biological and biochemical research. This compound is particularly valued for its ability to maintain stable pH levels in various experimental conditions, making it essential for cell culture, protein purification, and enzyme assays. Its unique structure allows for effective buffering in physiological pH ranges, which is crucial for maintaining the integrity of biological samples and reactions. Researchers often prefer HEPE over other buffers due to its low toxicity and compatibility with a wide range of biological systems, ensuring minimal interference with experimental outcomes.

In addition to its buffering capabilities, HEPE is also employed in the formulation of various pharmaceutical products and diagnostic reagents. Its solubility and stability in aqueous solutions enhance its utility in laboratory settings, where precise pH control is necessary. Furthermore, HEPE's role in stabilizing proteins and enzymes under experimental conditions highlights its importance in drug development and biotechnological applications. With its proven effectiveness and reliability, HEPE continues to be a preferred choice for researchers seeking to optimize their experimental protocols.

Numéro CAS 
7365-45-9
Formule moléculaire
C8H18N2O4S
Poids moléculaire 
238.3
Point de fusion 
234 - 235 °C
Informations générales
Numéro CAS 
7365-45-9
Formule moléculaire
C8H18N2O4S
Poids moléculaire 
238.3
Point de fusion 
234 - 235 °C
Propriétés
Informations complémentaires sur la propriété à venir prochainement !
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Sécurité et réglementation
Matières dangereuses
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Antibiotique
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Réglementé par la DEA
Non
Avertissements 
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Applications

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid is widely utilized in research focused on:

  • Biological Buffers: This compound is commonly used to create buffers in biological and biochemical research, helping to maintain pH stability in various experiments, which is crucial for enzyme activity and cell culture.
  • Pharmaceutical Formulations: It serves as an excipient in drug formulations, enhancing the solubility and stability of active pharmaceutical ingredients, thus improving the efficacy of medications.
  • Cell Culture Media: The chemical is incorporated into cell culture media, providing an optimal environment for the growth of various cell types, which is essential for research in biotechnology and pharmaceuticals.
  • Protein Purification: It is utilized in protein purification processes, helping to stabilize proteins during chromatography, thus ensuring higher yields and purity of the target proteins.
  • Analytical Chemistry: This compound is used in analytical methods, such as HPLC, to improve the resolution of complex mixtures, making it easier for researchers to analyze compounds accurately.

Citations