Naphthol blue black is widely utilized in research focused on:

• Histology and Cytology: This compound serves as a vital stain in histological studies, allowing researchers to visualize cellular structures and identify tissue types effectively.
• Environmental Monitoring: It is employed in water quality testing to detect and quantify heavy metals, providing crucial data for environmental assessments and regulatory compliance.
• Textile Industry: Naphthol blue black is used as a dye in textiles, offering vibrant colors and excellent fastness properties, which are essential for high-quality fabric production.
• Food Industry: It acts as a colorant in certain food products, enhancing visual appeal while meeting safety regulations, making it a popular choice among food manufacturers.
• Biochemical Research: The compound is utilized in various biochemical assays, particularly in the study of protein interactions and enzyme activities, aiding researchers in understanding complex biological processes.