Methylene blue is widely utilized in research focused on:

• Medical Diagnostics: Used as a dye in various diagnostic procedures, including staining tissues and cells to enhance visibility under a microscope, aiding in the detection of certain diseases.
• Antimicrobial Applications: Effective against a range of bacteria and fungi, making it valuable in developing antiseptic products and treatments for infections.
• Biological Research: Serves as a redox indicator in biochemical assays, helping researchers study cellular respiration and metabolic processes.
• Histology and Pathology: Commonly employed in histological staining to differentiate between various cell types, assisting pathologists in diagnosing conditions.
• Environmental Monitoring: Used in water treatment processes to detect and quantify the presence of certain contaminants, contributing to environmental safety and compliance.