Acridine orange hydrochloride solution, 10 mg/mL in H2O is widely utilized in research focused on:

• Cell Viability Assays: This compound is commonly used in laboratories to assess cell viability and cytotoxicity. By staining live and dead cells, researchers can easily differentiate between healthy and compromised cells, providing crucial data for drug development and toxicity studies.
• Fluorescence Microscopy: Its fluorescent properties make it an excellent choice for visualizing cellular structures and processes under a fluorescence microscope. This is particularly beneficial in fields like cell biology and microbiology, where understanding cellular dynamics is essential.
• DNA and RNA Staining: Acridine orange is effective for staining nucleic acids, allowing researchers to study genetic material in various organisms. This application is vital in genetics and molecular biology, enhancing the understanding of gene expression and cellular function.
• Histological Studies: In histology, this compound aids in the examination of tissue samples. Its ability to highlight specific cellular components helps pathologists and researchers identify abnormalities, contributing to disease diagnosis and research.
• Antimicrobial Research: The compound is also explored for its potential in antimicrobial studies. By evaluating its effects on microbial cells, researchers can investigate new therapeutic agents, which is crucial in combating antibiotic resistance.