Dihydroethidium is a versatile fluorescent compound widely utilized in biological and chemical research, particularly in the study of reactive oxygen species (ROS) and cellular oxidative stress. This compound is recognized for its ability to selectively stain live cells, allowing researchers to visualize and quantify oxidative stress in real-time. Its unique properties enable it to penetrate cell membranes and react with superoxide anions, producing a bright fluorescent signal that can be easily detected using fluorescence microscopy or flow cytometry.

In addition to its applications in oxidative stress research, Dihydroethidium has been employed in various fields, including pharmacology and toxicology, to assess the effects of drugs and environmental toxins on cellular health. Its specificity and sensitivity make it an invaluable tool for researchers aiming to understand the mechanisms of diseases associated with oxidative damage, such as neurodegenerative disorders and cancer. By providing clear insights into cellular processes, Dihydroethidium supports advancements in therapeutic strategies and drug development.