Methotrexate hydrate is widely utilized in research focused on:

• Cancer Treatment: This compound is a cornerstone in chemotherapy, particularly for treating various types of cancers such as leukemia and lymphoma. Its ability to inhibit cell growth makes it effective in targeting rapidly dividing cancer cells.
• Autoimmune Disorders: Methotrexate is commonly prescribed for conditions like rheumatoid arthritis and psoriasis. It helps reduce inflammation and suppress the immune system, providing relief to patients.
• Research in Biochemistry: In laboratory settings, it is used to study metabolic pathways and enzyme activity. Researchers utilize it to understand folate metabolism and its implications in cellular processes.
• Pharmaceutical Development: The compound serves as a model for developing new drugs. Its mechanism of action provides insights into designing more effective therapies with fewer side effects.
• Veterinary Medicine: Methotrexate is also applied in treating certain cancers and autoimmune diseases in animals, showcasing its versatility beyond human medicine.