Lissamine Green B is widely utilized in research focused on:

• Histology and Cytology: This dye is commonly used for staining tissues and cells, allowing researchers to visualize cellular structures under a microscope. Its strong affinity for cellular components enhances contrast, making it easier to identify abnormalities.
• Microbiology: In microbiological studies, Lissamine Green B serves as a vital stain for identifying and differentiating between bacterial species, aiding in the diagnosis of infections.
• Pharmaceutical Research: The compound is employed in drug formulation studies, particularly in assessing the permeability of drug compounds across biological membranes, which is crucial for developing effective medications.
• Environmental Monitoring: Lissamine Green B is used in assessing water quality, as it can indicate the presence of pollutants or harmful microorganisms, thus playing a role in environmental health and safety.
• Diagnostic Applications: In clinical settings, this dye is utilized in various diagnostic tests, including ophthalmic examinations, where it helps to visualize corneal defects and assess eye health.