Boc-O-tert-butyl-L-threonine is widely utilized in research focused on:

• Peptide Synthesis: This compound serves as a protecting group for amino acids during peptide synthesis, allowing for the selective modification of specific sites without affecting others. This is crucial in creating complex peptides for pharmaceuticals.
• Drug Development: It plays a significant role in the development of new therapeutic agents, particularly in the design of peptide-based drugs, enhancing their stability and bioavailability.
• Bioconjugation: The compound is used in bioconjugation processes, where it helps in attaching biomolecules to other molecules, improving the efficacy of drug delivery systems.
• Research in Protein Engineering: Researchers utilize it to modify proteins, allowing for the study of protein interactions and functions, which is vital in understanding diseases and developing treatments.
• Analytical Chemistry: It is employed as a standard in analytical methods, helping in the quantification and characterization of amino acids and peptides in various samples.