Digitonin is widely utilized in research focused on:

• Biochemical Research: It is commonly used as a tool for studying membrane proteins and their interactions, particularly in the isolation and purification of membrane-bound enzymes.
• Pharmaceutical Applications: Digitonin serves as a natural detergent in drug formulation, enhancing the solubility and bioavailability of poorly soluble compounds, which is crucial in developing effective medications.
• Cell Biology: Researchers employ digitonin to selectively permeabilize cell membranes, allowing for the study of intracellular processes without disrupting overall cell integrity.
• Analytical Chemistry: It is used in assays to quantify cholesterol levels in biological samples, providing a reliable method for assessing lipid metabolism and related health conditions.
• Plant Research: Digitonin helps in extracting and analyzing chlorophyll and other pigments from plant tissues, aiding in studies related to photosynthesis and plant health.