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Catalog Number:
22988
CAS Number:
150322-01-3
5-(N-Maleimido)fluorescein diacetate
Purity:
≥ 99% (NMR)
Synonym(s):
Fluorescein diacetate 5-maleimide
Documents
$299.45 /100MG
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Product Information

5-(N-Maleimido)fluorescein diacetate is a versatile fluorescent probe widely utilized in biochemical and cellular research. This compound features a maleimide functional group, which allows for selective labeling of thiol-containing biomolecules, making it an invaluable tool for studying protein interactions and cellular processes. Its fluorescent properties enable real-time imaging and tracking of biological events, providing researchers with insights into cellular dynamics and molecular mechanisms. The diacetate form enhances cell permeability, facilitating its use in live-cell imaging applications.

In addition to its applications in fluorescence microscopy, 5-(N-Maleimido)fluorescein diacetate is also employed in flow cytometry and various assays that require sensitive detection of biomolecules. Its unique ability to covalently bond with thiol groups allows for precise labeling, which is crucial in the development of targeted therapies and diagnostics. Researchers benefit from its high sensitivity and specificity, making it a preferred choice for studies involving protein localization and interaction analysis.

Synonyms
Fluorescein diacetate 5-maleimide
CAS Number
150322-01-3
Purity
≥ 99% (NMR)
Molecular Formula
C28H17NO9
Molecular Weight
511.44
MDL Number
MFCD00077346
PubChem ID
4561711
Melting Point
226-231 ?C
Appearance
Off-white to light yellow solid
Conditions
Store at 0-8 °C
General Information
Synonyms
Fluorescein diacetate 5-maleimide
CAS Number
150322-01-3
Purity
≥ 99% (NMR)
Molecular Formula
C28H17NO9
Molecular Weight
511.44
MDL Number
MFCD00077346
PubChem ID
4561711
Melting Point
226-231 ?C
Appearance
Off-white to light yellow solid
Conditions
Store at 0-8 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

5-(N-Maleimido)fluorescein diacetate is widely utilized in research focused on:

  • Cellular Imaging: This compound is commonly used as a fluorescent probe for visualizing live cells. Its ability to penetrate cell membranes allows researchers to study cellular processes in real-time, making it invaluable in cell biology.
  • Bioconjugation: The maleimide group in this compound facilitates the conjugation with thiol-containing biomolecules, such as proteins and peptides. This property is particularly useful in developing targeted drug delivery systems and creating biosensors.
  • Flow Cytometry: It serves as a fluorescent marker in flow cytometry applications, enabling the analysis of cell populations based on their fluorescence. This is essential for immunology and cancer research, where cell characterization is crucial.
  • Drug Development: In pharmaceutical research, it aids in tracking drug interactions within cells, providing insights into pharmacokinetics and drug efficacy. This can significantly streamline the drug discovery process.
  • Environmental Monitoring: The compound can be used in assays to detect and quantify pollutants in environmental samples, contributing to ecological studies and regulatory compliance.

Citations