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Catalog Number:
42535
CAS Number:
25535-16-4
Propidium iodide solution ((1.0 mg/ml in water))
Purity:
≥ 95% (HPLC)
Synonym(s):
3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodidemethiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium
Documents
$110.00 /10ML
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Product Information

Propidium iodide solution (1.0 mg/ml in water) is a vital fluorescent dye widely utilized in molecular biology and cell biology applications. Known for its ability to penetrate only dead or damaged cells, this compound is instrumental in distinguishing live cells from non-viable ones during fluorescence microscopy and flow cytometry. Its unique properties make it an essential tool for researchers studying cell viability, apoptosis, and cell cycle analysis. Propidium iodide binds to nucleic acids, emitting a strong fluorescence upon excitation, which enhances the visibility of cellular structures and processes.

In addition to its role in cell viability assays, propidium iodide is also used in various applications such as DNA staining, where it aids in the visualization of genetic material in agarose gels. Its compatibility with other fluorescent dyes allows for multiplexing in experiments, providing researchers with comprehensive data. The ease of use and reliable results make propidium iodide a preferred choice for laboratories focused on cell biology, cancer research, and drug development.

Synonyms
3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodidemethiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium
CAS Number
25535-16-4
Purity
≥ 95% (HPLC)
Molecular Formula
C27H34I2N4
Molecular Weight
668.39
MDL Number
MFCD00011921
PubChem ID
104981
Appearance
Orange red liquid
Conditions
Store at 2 - 8 °C
General Information
Synonyms
3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodidemethiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium
CAS Number
25535-16-4
Purity
≥ 95% (HPLC)
Molecular Formula
C27H34I2N4
Molecular Weight
668.39
MDL Number
MFCD00011921
PubChem ID
104981
Appearance
Orange red liquid
Conditions
Store at 2 - 8 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Propidium iodide solution (1.0 mg/ml in water) is widely utilized in research focused on:

  • Cell Viability Assays: This compound is commonly used to assess cell membrane integrity in various cell types, helping researchers determine the viability of cells in response to treatments or stress.
  • Flow Cytometry: Propidium iodide is a vital tool in flow cytometry for staining DNA in cells, allowing for the analysis of cell cycle phases and apoptosis in a high-throughput manner.
  • Fluorescence Microscopy: Its fluorescent properties make it ideal for visualizing dead cells in tissue samples, providing insights into cellular health and disease progression.
  • Gene Delivery Studies: Researchers use this solution to evaluate the efficiency of gene delivery systems by distinguishing between successfully transfected and non-transfected cells.
  • Pharmaceutical Development: In drug development, it helps in screening compounds for cytotoxicity, ensuring that new drugs are safe for human use by evaluating their effects on cell survival.

Citations