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Catalog Number:
00320
CAS Number:
7240-90-6
5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside
Purity:
≥ 98% (HPLC)
Synonym(s):
5-Bromo-4-chloro-3-indolyl-β-D-galactoside, X-Gal
Documents
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Product Information

5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside is a valuable synthetic compound widely utilized in molecular biology and biochemistry as a chromogenic substrate for the detection of β-galactosidase activity. This compound is particularly significant in the study of gene expression and protein function, providing researchers with a reliable means to visualize and quantify enzyme activity in various biological assays. Its unique structure allows for a clear colorimetric change upon hydrolysis by β-galactosidase, making it an essential tool in cloning experiments and the analysis of recombinant proteins.

In addition to its applications in research, 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside is also beneficial in the development of diagnostic assays and in the pharmaceutical industry for drug discovery processes. Its high sensitivity and specificity make it a preferred choice for scientists looking to enhance the accuracy of their experimental results. With its robust performance and versatility, this compound stands out as a critical reagent for both academic and industrial applications.

Synonyms
5-Bromo-4-chloro-3-indolyl-β-D-galactoside, X-Gal
CAS Number
7240-90-6
Purity
≥ 98% (HPLC)
Molecular Formula
C14H15BrClNO6
Molecular Weight
408.64
MDL Number
MFCD00005666
PubChem ID
619018
Melting Point
230 °C (dec.)
Appearance
White to off-white crystalline powder
Optical Rotation
[a]D20 = -60 to -64 º
Conditions
Store at ≤ -4 °C
General Information
Synonyms
5-Bromo-4-chloro-3-indolyl-β-D-galactoside, X-Gal
CAS Number
7240-90-6
Purity
≥ 98% (HPLC)
Molecular Formula
C14H15BrClNO6
Molecular Weight
408.64
MDL Number
MFCD00005666
PubChem ID
619018
Melting Point
230 °C (dec.)
Appearance
White to off-white crystalline powder
Optical Rotation
[a]D20 = -60 to -64 º
Conditions
Store at ≤ -4 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside is widely utilized in research focused on:

  • Biochemical Assays: This compound serves as a substrate in enzyme assays, particularly for β-galactosidase. It allows researchers to measure enzyme activity through colorimetric changes, providing a straightforward method for quantifying enzyme function.
  • Gene Expression Studies: It is commonly used in molecular biology to study gene expression. By incorporating this compound into experiments, scientists can track the activity of specific genes, aiding in the understanding of genetic regulation.
  • Plant Research: In plant biology, it helps in identifying and studying the function of genes involved in plant development and metabolism. This application is crucial for advancing agricultural biotechnology and crop improvement.
  • Drug Development: The compound is valuable in pharmaceutical research, particularly in screening for new drugs that target specific enzymes or pathways. Its ability to provide clear results makes it a preferred choice in early-stage drug discovery.
  • Diagnostics: In clinical settings, it can be used to develop diagnostic tests that detect enzyme deficiencies or abnormalities, offering a practical tool for medical research and patient care.

Citations