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Catalog Number:
21199
CAS Number:
93863-88-8
5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside
Purity:
≥ 99% (TLC)
Synonym(s):
5-Bromo-6-chloro-3-indoxyl-β-D-galactopyranoside, Magenta Gal
Documents
$65.40 /1G
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Product Information

5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside is a valuable compound widely utilized in biochemical research, particularly in the study of gene expression and enzyme activity. This compound serves as a chromogenic substrate for β-galactosidase, enabling researchers to visualize and quantify enzyme activity in various biological assays. Its unique structure, featuring both bromine and chlorine substituents, enhances its reactivity and stability, making it an ideal choice for applications in molecular biology and biochemistry.

In practical applications, 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside is often employed in histochemical staining procedures, allowing for the detection of β-galactosidase in tissue samples. This capability is particularly beneficial in developmental biology and cancer research, where understanding gene expression patterns is crucial. Additionally, its ease of use and reliable performance compared to similar substrates make it a preferred choice among researchers seeking accurate and reproducible results in their experiments.

Synonyms
5-Bromo-6-chloro-3-indoxyl-β-D-galactopyranoside, Magenta Gal
CAS Number
93863-88-8
Purity
≥ 99% (TLC)
Molecular Formula
C14H15BrClNO6
Molecular Weight
408.63
MDL Number
MFCD00216824
PubChem ID
4131401
Appearance
White to off-white powder
Optical Rotation
[a]D20 = -45 ± 2º (dry basis)
Conditions
Store at 0-8 °C
General Information
Synonyms
5-Bromo-6-chloro-3-indoxyl-β-D-galactopyranoside, Magenta Gal
CAS Number
93863-88-8
Purity
≥ 99% (TLC)
Molecular Formula
C14H15BrClNO6
Molecular Weight
408.63
MDL Number
MFCD00216824
PubChem ID
4131401
Appearance
White to off-white powder
Optical Rotation
[a]D20 = -45 ± 2º (dry basis)
Conditions
Store at 0-8 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

5-Bromo-6-chloro-3-indolyl-b-D-galactopyranoside is widely utilized in research focused on:

  • Biochemical Assays: This compound serves as a substrate in enzyme assays, particularly for β-galactosidase. Its use allows researchers to measure enzyme activity, which is crucial in understanding metabolic pathways.
  • Cell Biology: It is employed in cell culture studies to visualize gene expression. When metabolized by cells, it produces a colored product, making it easier to track cellular processes and responses.
  • Drug Development: The compound is valuable in pharmaceutical research for screening potential drug candidates. Its ability to indicate enzyme activity can help identify compounds that modulate specific biological pathways.
  • Microbiology: In microbiological studies, it acts as an indicator for the presence of certain bacteria. This application is particularly useful in food safety testing and environmental monitoring.
  • Genetic Engineering: It is used in molecular biology techniques, such as cloning and gene expression studies. By providing a visual marker, it helps researchers confirm successful genetic modifications.

Citations