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Catalog Number:
01474
CAS Number:
596-09-8
Fluorescein diacetate
Purity:
≥ 99% (Assay, dry basis)
Synonym(s):
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
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Product Information

Fluorescein diacetate is a highly versatile fluorescent dye widely utilized in biological and chemical research. This compound is particularly valued for its ability to permeate cell membranes, making it an excellent indicator for assessing cell viability and metabolic activity. Upon entering viable cells, fluorescein diacetate is hydrolyzed by intracellular esterases, releasing fluorescein, which emits a bright green fluorescence. This property is leveraged in various applications, including live-cell imaging, cytotoxicity assays, and flow cytometry, allowing researchers to monitor cellular processes in real-time.

In addition to its applications in cell biology, fluorescein diacetate is also employed in environmental studies to trace water movement and assess the bioavailability of pollutants. Its unique ability to provide clear, quantifiable fluorescence makes it a preferred choice over other dyes in many experimental setups. Researchers and industry professionals benefit from its ease of use and reliable performance, ensuring accurate results in both laboratory and field applications.

Synonyms
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
CAS Number
596-09-8
Purity
≥ 99% (Assay, dry basis)
Molecular Formula
C24H16O7
Molecular Weight
416.4
MDL Number
MFCD00005062
PubChem ID
65047
Melting Point
201-207 ºC
Appearance
Light yellow crystalline powder
Conditions
Store at 0-8 °C
General Information
Synonyms
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
CAS Number
596-09-8
Purity
≥ 99% (Assay, dry basis)
Molecular Formula
C24H16O7
Molecular Weight
416.4
MDL Number
MFCD00005062
PubChem ID
65047
Melting Point
201-207 ºC
Appearance
Light yellow crystalline powder
Conditions
Store at 0-8 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Fluorescein diacetate is widely utilized in research focused on:

  • Cell Viability Assays: This compound is commonly used in laboratories to assess cell health and viability. It can easily penetrate live cells and is converted into a fluorescent product, allowing researchers to quantify living cells in various biological studies.
  • Fluorescent Microscopy: Its strong fluorescence makes it ideal for imaging applications. Researchers in fields like cell biology and pathology use it to visualize cellular structures and processes, providing clear insights into cellular functions.
  • Drug Development: In pharmaceutical research, fluorescein diacetate helps in screening potential drug candidates by evaluating their effects on cell viability and proliferation, streamlining the drug discovery process.
  • Environmental Monitoring: This compound can be utilized to track microbial activity in environmental samples, aiding in studies related to soil health and water quality, which is crucial for ecological research.
  • Histological Studies: In histology, fluorescein diacetate is employed to stain tissues, enhancing the visualization of specific cellular components, which is essential for accurate diagnosis and research in medical fields.

Citations