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Catalog Number:
00287
CAS Number:
54827-17-7
3,3',5,5'-Tetramethylbenzidine
Purity:
≥ 99% (Assay)
Synonym(s):
TMB
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Product Information

3,3',5,5'-Tetramethylbenzidine is a versatile organic compound widely recognized for its application as a chromogenic substrate in various biochemical assays. This compound is particularly valued in the field of immunohistochemistry and enzyme-linked immunosorbent assays (ELISA) due to its ability to produce a distinct color change upon reaction with peroxidase enzymes. Its high sensitivity and specificity make it an ideal choice for researchers looking to enhance the detection of antigens in complex biological samples.

In addition to its use in diagnostic applications, 3,3',5,5'-Tetramethylbenzidine is also employed in the production of dyes and pigments, providing vibrant colors in various industrial applications. Its unique chemical structure allows for stability and reactivity, making it a preferred choice for professionals in both research and manufacturing sectors. With its proven track record in enhancing assay performance and its adaptability in various formulations, this compound stands out as a valuable tool for scientists and industry experts alike.

Synonyms
TMB
CAS Number
54827-17-7
Purity
≥ 99% (Assay)
Molecular Formula
C16H20N2
Molecular Weight
240.35
MDL Number
MFCD00007748
PubChem ID
41206
Melting Point
168 - 171 °C
Appearance
White to pale white powder
Conditions
Store at RT
General Information
Synonyms
TMB
CAS Number
54827-17-7
Purity
≥ 99% (Assay)
Molecular Formula
C16H20N2
Molecular Weight
240.35
MDL Number
MFCD00007748
PubChem ID
41206
Melting Point
168 - 171 °C
Appearance
White to pale white powder
Conditions
Store at RT
Properties
Additional property information coming soon!
-
Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

3,3',5,5'-Tetramethylbenzidine is widely utilized in research focused on:

  • Colorimetric Assays: This compound is commonly used as a substrate in enzyme-linked immunosorbent assays (ELISAs), where it produces a measurable color change, allowing for the quantification of biomolecules.
  • Histochemistry: It serves as a chromogen in histological staining, helping researchers visualize cellular components under a microscope, particularly in tissue samples.
  • Diagnostic Testing: Its application in diagnostic kits for various diseases enhances the sensitivity and specificity of tests, making it valuable in clinical laboratories.
  • Environmental Monitoring: The compound is utilized in detecting trace amounts of pollutants in environmental samples, aiding in the assessment of water and soil quality.
  • Research and Development: It plays a critical role in the development of new analytical methods and technologies, providing a reliable option for scientists in various fields, including biochemistry and molecular biology.

Citations