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Catalog Number:
00194
CAS Number:
367-93-1
Isopropyl-β-D-thiogalactopyranoside, dioxane free
Purity:
≥ 98% (HPLC)
Synonym(s):
IPTG, Isopropyl-β-D-thiogalactoside
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Product Information

Isopropyl-b-D-thiogalactopyranoside, dioxane free, is a versatile compound widely utilized in molecular biology and biochemistry as a potent inducer of gene expression. This compound serves as a valuable tool for researchers working with systems that require controlled expression of proteins, particularly in the context of recombinant DNA technology. Its unique structure allows for effective interaction with various biological systems, making it an essential reagent for studies involving lactose operon systems, especially in E. coli.

The dioxane-free formulation of Isopropyl-b-D-thiogalactopyranoside enhances its appeal by minimizing potential solvent-related toxicity, thereby ensuring safer handling and application in laboratory settings. Researchers can leverage this compound for applications such as protein purification, enzyme assays, and functional studies of gene expression, providing a reliable means to achieve desired experimental outcomes. Its compatibility with a range of biological assays further underscores its significance in advancing molecular research and biotechnology applications.

Synonyms
IPTG, Isopropyl-β-D-thiogalactoside
CAS Number
367-93-1
Purity
≥ 98% (HPLC)
Molecular Formula
C9H18O5S
Molecular Weight
238.31
MDL Number
MFCD00063273
PubChem ID
552632
Melting Point
108 - 116 °C
Appearance
White to almost white crystalline powder
Optical Rotation
[a]20D = -28.5 to -34.5 °
Conditions
Store at 0 - 8 °C
General Information
Synonyms
IPTG, Isopropyl-β-D-thiogalactoside
CAS Number
367-93-1
Purity
≥ 98% (HPLC)
Molecular Formula
C9H18O5S
Molecular Weight
238.31
MDL Number
MFCD00063273
PubChem ID
552632
Melting Point
108 - 116 °C
Appearance
White to almost white crystalline powder
Optical Rotation
[a]20D = -28.5 to -34.5 °
Conditions
Store at 0 - 8 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Isopropyl-b-D-thiogalactopyranoside, dioxane free is widely utilized in research focused on:

  • Biotechnology: It serves as an important inducer for the expression of recombinant proteins in bacterial systems, particularly in E. coli, enhancing the yield of target proteins.
  • Gene Expression Studies: This compound is crucial in studies involving gene regulation, allowing researchers to control the expression of specific genes in various experimental setups.
  • Pharmaceutical Development: It is used in drug discovery processes, particularly in screening assays to identify compounds that can modulate biological pathways effectively.
  • Cell Biology: The chemical is utilized in cell culture applications, helping to investigate cellular responses and signaling pathways in response to various stimuli.
  • Enzyme Activity Assays: It acts as a substrate in enzymatic reactions, aiding researchers in studying enzyme kinetics and mechanisms, which is essential for understanding metabolic pathways.

Citations