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Catalog Number:
47748
CAS Number:
4091-99-0
2′,7′-Dichlorofluorescin diacetate
Purity:
≥ 97% (HPLC)
Synonym(s):
2',7'-Dichlorodihydrofluorescein diacetate
Documents
$120.00 /100MG
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Product Information

2',7'-Dichlorofluorescin diacetate is a versatile fluorescent dye widely utilized in biological and chemical research. This compound serves as a cell-permeable indicator for reactive oxygen species (ROS) and is particularly effective in detecting oxidative stress in live cells. Upon entering the cell, it is hydrolyzed by intracellular esterases to release the highly fluorescent 2',7'-dichlorofluorescein, which can be quantified using fluorescence microscopy or flow cytometry. This makes it an invaluable tool for researchers studying cellular processes, drug effects, and the mechanisms of diseases associated with oxidative stress.

In addition to its applications in cell biology, 2',7'-Dichlorofluorescin diacetate is also employed in various assays to evaluate the antioxidant capacity of compounds and to monitor the efficacy of therapeutic agents. Its high sensitivity and specificity for ROS make it a preferred choice in pharmacological studies and toxicology assessments. Researchers can leverage this compound to gain insights into cellular health and the impact of environmental factors on biological systems.

Synonyms
2',7'-Dichlorodihydrofluorescein diacetate
CAS Number
4091-99-0
Purity
≥ 97% (HPLC)
Molecular Formula
C24H16Cl2O7
Molecular Weight
487.29
MDL Number
MFCD00128955
Melting Point
203 - 207 °C
Appearance
White to off-white or yellow powder
Conditions
Store at -20 °C
General Information
Synonyms
2',7'-Dichlorodihydrofluorescein diacetate
CAS Number
4091-99-0
Purity
≥ 97% (HPLC)
Molecular Formula
C24H16Cl2O7
Molecular Weight
487.29
MDL Number
MFCD00128955
Melting Point
203 - 207 °C
Appearance
White to off-white or yellow powder
Conditions
Store at -20 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications
2',7'-Dichlorofluorescin diacetate is widely utilized in research focused on
  • Cell Viability Assays: This compound is commonly used in assays to assess cell viability and proliferation. It can be converted into a fluorescent product, allowing researchers to quantify living cells in various biological studies.
  • Reactive Oxygen Species (ROS) Detection: It serves as a probe for detecting reactive oxygen species in cells. This application is crucial in studies related to oxidative stress and cellular responses to different stimuli.
  • Drug Development: In pharmaceutical research, it is used to evaluate the efficacy of new drugs by monitoring their effects on cellular health and oxidative stress levels, providing insights into potential therapeutic benefits.
  • Fluorescence Microscopy: The compound is utilized in fluorescence microscopy to visualize cellular processes in real-time. Its fluorescent properties allow for detailed imaging of cellular structures and functions.
  • Environmental Monitoring: It can be applied in environmental science to assess the impact of pollutants on microbial communities, helping researchers understand the effects of contaminants on ecosystem health.

Citations