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Catalog Number:
30011
CAS Number:
79955-27-4
5-Carboxyfluorescein diacetate
Purity:
≥ 98% (HPLC)
Synonym(s):
5-Carboxy-di-O-acetylfluorescein
Documents
$65.40 /25MG
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Product Information

5-Carboxyfluorescein diacetate is a versatile fluorescent dye widely utilized in biological and chemical research. This compound is recognized for its ability to permeate cell membranes, making it an invaluable tool for cell viability assays and tracking cellular processes. Upon entering the cell, it is hydrolyzed by intracellular esterases to release the fluorescent 5-carboxyfluorescein, which can be detected using fluorescence microscopy or flow cytometry. This property allows researchers to study cell health, proliferation, and apoptosis in real-time, providing insights into various biological processes.

Additionally, 5-Carboxyfluorescein diacetate is employed in various applications, including drug delivery studies and the development of biosensors. Its high sensitivity and specificity make it an excellent choice for detecting specific biomolecules in complex samples. Researchers appreciate its stability and ease of use, which facilitate a wide range of experimental designs. With its unique properties, this compound stands out as a crucial reagent in the toolkit of scientists focused on cellular and molecular biology.

Synonyms
5-Carboxy-di-O-acetylfluorescein
CAS Number
79955-27-4
Purity
≥ 98% (HPLC)
Molecular Formula
C25H16O9
Molecular Weight
460.39
MDL Number
MFCD00036872
PubChem ID
133314
Appearance
White powder
Conditions
Store at ≤ - 20 °C
General Information
Synonyms
5-Carboxy-di-O-acetylfluorescein
CAS Number
79955-27-4
Purity
≥ 98% (HPLC)
Molecular Formula
C25H16O9
Molecular Weight
460.39
MDL Number
MFCD00036872
PubChem ID
133314
Appearance
White powder
Conditions
Store at ≤ - 20 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

5-Carboxyfluorescein diacetate is widely utilized in research focused on:

  • Cell Viability Assays: This compound is commonly used in assays to assess cell health and viability. It can easily penetrate live cells and is converted to a fluorescent form, allowing researchers to quantify living cells in various biological studies.
  • Fluorescent Tracing: In cellular and molecular biology, it serves as a tracer for studying cellular uptake and distribution. Its fluorescence properties enable visualization of cellular processes, making it invaluable in drug delivery research.
  • Environmental Monitoring: The compound is applied in environmental science to track the presence of pollutants. Its fluorescent nature allows for sensitive detection of contaminants in water samples, aiding in ecological assessments.
  • Imaging Techniques: It is used in various imaging techniques, including fluorescence microscopy. This application helps researchers visualize cellular structures and dynamics in real-time, enhancing the understanding of biological mechanisms.
  • Biochemical Research: The compound is instrumental in biochemical assays for enzyme activity and metabolic studies. Its ability to indicate changes in cellular conditions provides insights into metabolic pathways and enzyme functions.

Citations