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Catalog Number:
38786
CAS Number:
124763-51-5
Bis-tris hydrochloride
Purity:
≥ 99% (Assay by titration)
Synonym(s):
2,2-Bis(hydroxymethyl)-2,2',2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
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Product Information

Bis-tris hydrochloride is a versatile buffering agent widely used in biological and biochemical research. This compound is particularly valued for its ability to maintain stable pH levels in various experimental conditions, making it ideal for applications in molecular biology, protein purification, and enzyme assays. Its unique structure allows it to effectively stabilize pH in the physiological range, which is crucial for maintaining the activity of sensitive biomolecules. Researchers appreciate its low UV absorbance, which minimizes interference in spectrophotometric measurements, and its compatibility with a wide range of biological systems.

In addition to its buffering capabilities, Bis-tris hydrochloride is often employed in the preparation of gels for electrophoresis, particularly in the separation of proteins and nucleic acids. Its effectiveness in these applications is enhanced by its ability to provide a consistent environment, ensuring reproducibility and reliability in experimental results. This compound is also recognized for its low toxicity, making it a safer alternative to other buffering agents, thus appealing to researchers focused on minimizing hazardous materials in their laboratories.

Synonyms
2,2-Bis(hydroxymethyl)-2,2',2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
CAS Number
124763-51-5
Purity
≥ 99% (Assay by titration)
Molecular Formula
C8H20ClNO5
Molecular Weight
245.7
MDL Number
MFCD00171252
PubChem ID
16219041
Appearance
White crystalline powder
Conditions
Store at RT
General Information
Synonyms
2,2-Bis(hydroxymethyl)-2,2',2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
CAS Number
124763-51-5
Purity
≥ 99% (Assay by titration)
Molecular Formula
C8H20ClNO5
Molecular Weight
245.7
MDL Number
MFCD00171252
PubChem ID
16219041
Appearance
White crystalline powder
Conditions
Store at RT
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Bis-tris hydrochloride is widely utilized in research focused on:

  • Biological Buffers: It serves as an effective buffering agent in biological and biochemical experiments, maintaining pH stability in various assays, which is crucial for enzyme activity and cell culture.
  • Protein Purification: This compound is often used in protein purification processes, particularly in chromatography, where it helps to stabilize proteins and improve yield during purification.
  • Electrophoresis: In molecular biology, it is commonly used in electrophoresis techniques, providing a consistent pH environment that enhances the resolution of nucleic acids and proteins during separation.
  • Pharmaceutical Formulations: Bis-tris hydrochloride is utilized in the formulation of various pharmaceuticals, ensuring the stability and efficacy of active ingredients in drug development.
  • Research and Development: It plays a significant role in R&D labs for developing new biochemical assays and testing protocols, especially in studies involving enzymes and metabolic pathways.

Citations