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Catalog Number:
38765
CAS Number:
7365-45-9
HEPES
Grade:
Suitable for cell culture
Purity:
≥ 99.5% (Assay by titration)
Synonym(s):
N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
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Product Information

HEPES, or 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, is a widely used buffering agent in biological and biochemical research. Its unique ability to maintain stable pH levels in physiological conditions makes it an essential component in cell culture media, protein purification, and enzyme assays. HEPES is particularly valued for its low toxicity and minimal interference with biological processes, which enhances the reliability of experimental results. Researchers often prefer HEPES over traditional buffers like phosphate due to its superior performance in maintaining pH stability in a variety of applications, including molecular biology, microbiology, and biochemistry.

In addition to its buffering capabilities, HEPES is also utilized in various pharmaceutical formulations and diagnostic assays, where precise pH control is critical. Its compatibility with a wide range of biological systems makes it an ideal choice for applications in drug development and vaccine research. With its proven track record in enhancing experimental accuracy and reproducibility, HEPES continues to be a go-to reagent for scientists and industry professionals alike.

Synonyms
N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
CAS Number
7365-45-9
Purity
≥ 99.5% (Assay by titration)
Grade
Suitable for cell culture
Molecular Formula
C8H18N2O4S
Molecular Weight
238.3
MDL Number
MFCD00006158
PubChem ID
23830
Melting Point
235 - 237 °C (dec.)
Appearance
White powder
Boiling Point
209 - 211 °C
Conditions
Store at RT
General Information
Synonyms
N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
CAS Number
7365-45-9
Purity
≥ 99.5% (Assay by titration)
Grade
Suitable for cell culture
Molecular Formula
C8H18N2O4S
Molecular Weight
238.3
MDL Number
MFCD00006158
PubChem ID
23830
Melting Point
235 - 237 °C (dec.)
Appearance
White powder
Boiling Point
209 - 211 °C
Conditions
Store at RT
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

HEPES is widely utilized in research focused on various applications that enhance scientific understanding and practical outcomes.

  • Cell Culture Media: HEPES is commonly used as a buffering agent in cell culture media, helping to maintain stable pH levels, which is crucial for cell viability and function.
  • Biochemical Assays: It serves as a key component in biochemical assays, providing a controlled environment that ensures accurate measurements of enzyme activity and other biochemical reactions.
  • Pharmaceutical Formulations: HEPES is incorporated into pharmaceutical formulations to stabilize active ingredients, improving the efficacy and shelf-life of medications.
  • Protein Purification: In protein purification processes, HEPES helps to maintain optimal conditions, enhancing the yield and purity of proteins, which is vital for research and therapeutic applications.
  • Biotechnology Applications: Its role in biotechnology, particularly in the production of monoclonal antibodies and vaccines, highlights its importance in developing new therapies and diagnostics.

Citations