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Catalog Number:
38757
CAS Number:
6976-37-0
Bis-tris
Grade:
suitable for cell culture
Purity:
≥ 99% (Assay by titration , HPLC)
Synonym(s):
["2, 2-Bis(hydroxymethyl)-2, 2', 2"-nitrilotriethanol, 2-Bis[2-hydroxyethyl]amino-2-[hydroxymethyl]1, 3-propanediol"]
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$64.50 /25G
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Product Information

Bis-tris is a versatile buffering agent widely utilized in biochemical and molecular biology applications. Its unique structure, featuring two hydroxyethyl groups and a hydroxymethyl group, allows it to maintain a stable pH in various biological systems, making it an essential component in protein purification, enzyme assays, and cell culture media. Researchers appreciate its low absorbance in the UV range, which minimizes interference in spectrophotometric measurements, thus enhancing the accuracy of experimental results.

In addition to its buffering capabilities, Bis-tris is known for its compatibility with a wide range of biological molecules, making it ideal for applications in electrophoresis and chromatography. Its ability to stabilize proteins and nucleic acids under varying conditions further underscores its importance in laboratory settings. With its favorable properties, Bis-tris stands out as a reliable choice for researchers seeking to optimize their experimental protocols and achieve reproducible results.

Synonyms
["2, 2-Bis(hydroxymethyl)-2, 2', 2"-nitrilotriethanol, 2-Bis[2-hydroxyethyl]amino-2-[hydroxymethyl]1, 3-propanediol"]
CAS Number
6976-37-0
Purity
≥ 99% (Assay by titration , HPLC)
Grade
suitable for cell culture
Molecular Formula
C8H19NO5
Molecular Weight
209.24
MDL Number
MFCD00002853
PubChem ID
81462
Melting Point
164 - 165 °C
Density
1.300
Appearance
White crystalline powder
Boiling Point
572 - 574 °C
Conditions
Store at RT
General Information
Synonyms
["2, 2-Bis(hydroxymethyl)-2, 2', 2"-nitrilotriethanol, 2-Bis[2-hydroxyethyl]amino-2-[hydroxymethyl]1, 3-propanediol"]
CAS Number
6976-37-0
Purity
≥ 99% (Assay by titration , HPLC)
Grade
suitable for cell culture
Molecular Formula
C8H19NO5
Molecular Weight
209.24
MDL Number
MFCD00002853
PubChem ID
81462
Melting Point
164 - 165 °C
Density
1.300
Appearance
White crystalline powder
Boiling Point
572 - 574 °C
Conditions
Store at RT
Properties
Additional property information coming soon!
-
Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Bis-tris is widely utilized in research focused on:

  • Biochemical Buffers: Commonly used as a buffering agent in biological and biochemical experiments, it helps maintain pH stability in various solutions, which is crucial for enzyme activity and protein stability.
  • Cell Culture Media: Essential in formulating cell culture media, it supports the growth of various cell types by providing an optimal environment, making it invaluable in pharmaceutical and biotechnology industries.
  • Electrophoresis: Frequently employed in electrophoresis applications, it aids in the separation of biomolecules, such as proteins and nucleic acids, allowing researchers to analyze complex mixtures effectively.
  • Protein Purification: Used in affinity chromatography, it enhances the purification process of proteins, ensuring high yield and purity, which is vital for drug development and research.
  • Pharmaceutical Formulations: Incorporated into various pharmaceutical formulations, it helps stabilize active ingredients, improving the efficacy and shelf-life of medications.

Citations