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Catalog Number:
37111
CAS Number:
7365-45-9
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
Grade:
Molecular biology grade
Purity:
99 - 101% (Assay by titration)
Synonym(s):
HEPES
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$29.34 /100G
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Product Information

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, commonly known as HEPE, is a versatile buffering agent widely utilized in biological and biochemical research. This compound is particularly valued for its ability to maintain stable pH levels in various experimental conditions, making it essential for cell culture, protein purification, and enzyme activity studies. HEPE is known for its excellent solubility in water and compatibility with a range of biological systems, which enhances its effectiveness in maintaining physiological pH. Researchers often prefer HEPE over other buffering agents due to its minimal interference with biological processes, ensuring accurate and reliable results in experiments.

In addition to its buffering capabilities, HEPE serves as a crucial component in the formulation of various pharmaceutical products and diagnostic assays. Its unique structure allows for effective stabilization of proteins and nucleic acids, which is vital in applications such as drug development and molecular biology. With its broad range of applications and superior performance, HEPE stands out as an indispensable tool for researchers and industry professionals seeking reliable and efficient solutions in their work.

Synonyms
HEPES
CAS Number
7365-45-9
Purity
99 - 101% (Assay by titration)
Grade
Molecular biology grade
Molecular Formula
C8H18N2O4S
Molecular Weight
238.3
MDL Number
MFCD00006158
PubChem ID
23830
Appearance
White powder
Conditions
Store at RT
General Information
Synonyms
HEPES
CAS Number
7365-45-9
Purity
99 - 101% (Assay by titration)
Grade
Molecular biology grade
Molecular Formula
C8H18N2O4S
Molecular Weight
238.3
MDL Number
MFCD00006158
PubChem ID
23830
Appearance
White powder
Conditions
Store at RT
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid is widely utilized in research focused on:

  • Biological Buffering: This compound is commonly used as a buffering agent in biological and biochemical applications, helping to maintain stable pH levels in various laboratory experiments, which is crucial for enzyme activity and cell culture.
  • Pharmaceutical Formulations: It serves as an excipient in drug formulations, enhancing the solubility and stability of active pharmaceutical ingredients, thereby improving the efficacy of medications.
  • Cell Culture Media: Frequently incorporated into cell culture media, it supports the growth of various cell types by providing a stable environment, making it essential for research in cellular biology and tissue engineering.
  • Protein Purification: This chemical is used in protein purification processes, particularly in chromatography, where it helps to maintain the desired pH and ionic strength, leading to higher yields and better purity of proteins.
  • Biotechnology Applications: It plays a role in various biotechnological applications, including the production of monoclonal antibodies and recombinant proteins, where precise pH control is vital for optimal yields.

Citations