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Catalog Number:
38567
CAS Number:
3326-35-0
5-Nitrofluorescein (isomer I)
Purity:
≥ 98% (Assay by titration)
Synonym(s):
5-Nitro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one(isomer I)
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Product Information

5-Nitrofluorescein (isomer I) is a highly versatile fluorescent dye widely utilized in various scientific and industrial applications. Known for its vibrant yellow-green fluorescence, this compound is particularly valuable in biological research, where it serves as a vital marker in fluorescence microscopy and flow cytometry. Its ability to bind to proteins and nucleic acids makes it an excellent choice for tracking cellular processes and visualizing biological structures. Additionally, 5-Nitrofluorescein is employed in environmental monitoring, particularly in assessing water quality, as it can indicate the presence of contaminants through fluorescence detection.

The unique properties of 5-Nitrofluorescein, such as its high photostability and sensitivity, set it apart from other fluorescent dyes, making it an ideal candidate for long-term imaging studies. Researchers appreciate its ease of use and compatibility with various solvents and buffers, allowing for flexibility in experimental design. Whether in academic research or industrial applications, 5-Nitrofluorescein continues to be an essential tool for scientists seeking reliable and effective fluorescent labeling solutions.

Synonyms
5-Nitro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one(isomer I)
CAS Number
3326-35-0
Purity
≥ 98% (Assay by titration)
Molecular Formula
C20H11NO7
Molecular Weight
377.31
MDL Number
MFCD00135108
PubChem ID
76846
Appearance
Light yellow to brown powder to crystal
Conditions
Store at RT
General Information
Synonyms
5-Nitro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one(isomer I)
CAS Number
3326-35-0
Purity
≥ 98% (Assay by titration)
Molecular Formula
C20H11NO7
Molecular Weight
377.31
MDL Number
MFCD00135108
PubChem ID
76846
Appearance
Light yellow to brown powder to crystal
Conditions
Store at RT
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

5-Nitrofluorescein (isomer I) is widely utilized in research focused on:

  • Fluorescent Dyes: This compound serves as a highly effective fluorescent dye in biological imaging, allowing researchers to visualize cellular processes with precision.
  • Environmental Monitoring: It is used in water testing to detect contaminants, as its fluorescence can indicate the presence of pollutants, aiding in environmental protection efforts.
  • pH Indicators: The chemical acts as a pH indicator due to its sensitivity to changes in acidity, making it valuable in laboratory experiments that require precise pH measurements.
  • Photodynamic Therapy: In medical applications, it shows promise in photodynamic therapy for cancer treatment, where its fluorescent properties help target and destroy cancer cells.
  • Research in Biochemistry: It is employed in various biochemical assays, enhancing the detection of proteins and nucleic acids, thus facilitating advancements in molecular biology.

Citations