Propidium iodide is a vital chemical compound widely utilized in molecular biology and cell biology applications. Known for its ability to intercalate with nucleic acids, it serves as a powerful fluorescent dye for staining dead cells in various assays, including flow cytometry and fluorescence microscopy. This compound is particularly effective in distinguishing live from dead cells, making it an essential tool in apoptosis studies and cell viability assessments. Its unique properties allow researchers to visualize cellular structures and monitor cellular processes, enhancing the understanding of complex biological systems.

In addition to its applications in cell viability assays, propidium iodide is also employed in DNA and RNA analysis, providing insights into genetic material integrity. Its compatibility with other fluorescent dyes allows for multiplexing in experiments, thereby increasing the efficiency of data collection. Researchers benefit from its high sensitivity and specificity, which facilitate accurate results in various experimental setups. With its broad range of applications in research and diagnostics, propidium iodide continues to be an indispensable resource for professionals in the life sciences.